import json with open("/etc/ssowat/conf.json.persistent", "r", encoding='utf-8') as jsonFile: data = json.load(jsonFile) data["protected_urls"].remove("__DOMAIN____PATH__/admin") with open("/etc/ssowat/conf.json.persistent", "w", encoding='utf-8') as jsonFile: jsonFile.write(json.dumps(data, indent=4, sort_keys=True))