diff --git a/data/helpers.d/setting b/data/helpers.d/setting
index af52b8321..d1babd140 100644
--- a/data/helpers.d/setting
+++ b/data/helpers.d/setting
@@ -78,7 +78,7 @@ ynh_app_setting_delete() {
 #
 ynh_app_setting()
 {
-    ACTION="$1" APP="$2" KEY="$3" VALUE="${4:-}" python2.7 - <<EOF
+    ACTION="$1" APP="$2" KEY="$3" VALUE="${4:-}" python3 - <<EOF
 import os, yaml, sys
 app, action = os.environ['APP'], os.environ['ACTION'].lower()
 key, value = os.environ['KEY'], os.environ.get('VALUE', None)
diff --git a/data/helpers.d/utils b/data/helpers.d/utils
index 3ab56747e..d1f5e16b7 100644
--- a/data/helpers.d/utils
+++ b/data/helpers.d/utils
@@ -421,7 +421,7 @@ ynh_render_template() {
     local output_path=$2
     mkdir -p "$(dirname $output_path)"
     # Taken from https://stackoverflow.com/a/35009576
-    python2.7 -c 'import os, sys, jinja2; sys.stdout.write(
+    python3 -c 'import os, sys, jinja2; sys.stdout.write(
                     jinja2.Template(sys.stdin.read()
                     ).render(os.environ));' < $template_path > $output_path
 }
diff --git a/data/hooks/conf_regen/01-yunohost b/data/hooks/conf_regen/01-yunohost
index c4120d487..6ac61d07a 100755
--- a/data/hooks/conf_regen/01-yunohost
+++ b/data/hooks/conf_regen/01-yunohost
@@ -115,7 +115,7 @@ do_post_regen() {
 }
 
 _update_services() {
-  python2 - << EOF
+  python3 - << EOF
 import yaml
 
 
diff --git a/debian/rules b/debian/rules
index 8afe372b5..54ebc5781 100755
--- a/debian/rules
+++ b/debian/rules
@@ -5,7 +5,7 @@
 #export DH_VERBOSE=1
 
 %:
-	dh ${@} --with=python2,systemd
+	dh ${@} --with=python3,systemd
 
 override_dh_auto_build:
 	# Generate bash completion file
diff --git a/doc/generate_helper_doc.py b/doc/generate_helper_doc.py
index bc9611c8f..908d6300e 100644
--- a/doc/generate_helper_doc.py
+++ b/doc/generate_helper_doc.py
@@ -1,4 +1,4 @@
-#!/usr/env/python2.7
+#!/usr/env/python3
 
 import os
 import glob